CeRVIM Seminar: Simultaneous fluorophore discrimination and resolution improvement of super-resolution images using fluorescence lifetime
Andréanne Deschênes
CERVO Research Centre, Université Laval
Friday, April 28, 2023, 11 a.m., PLT-3370
Abstract
To study the interactions between neuronal proteins with fluorescence microscopy, simultaneous observation of multiple biological markers is required. SPLIT-STED, an approach exploiting the analysis of fluorescence lifetime was developed to improve the spatial resolution of STimulated Emission Depletion microscopy. We developed an analysis using linear combination of components in phasor space to multiplex SPLIT-STED and apply it to separate two spectrally indistinguishable fluorophores per imaging channel. We quantify and characterize the performance of our algorithm on simulated images constructed from real single-staining images.This allows us to perform simultaneous resolution improvement and colocalization analysis of multiple protein species in live and fixed neuronal cultures.
The presentation will be given in English and the slides will be in English.